Cisplatin zebrafish

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August undefined, 2024

WebNov 18, 2024 · Cisplatin is an effective anticancer agent, but also causes permanent hearing loss by damaging hair cells-the sensory receptors essential for hearing. There is an urgent clinical need to protect cochlear hair cells in … WebSep 1, 2024 · Zebrafish embryos were exposed to cisplatin (0, 50, and 100 μM) for 96 h (4–100 h post-fertilization) and then stained with fluorescent dyes to reveal mitochondrial activity (rhodamine123), apoptosis (acridine orange), and oxidative stress (CellROX/MitoSOX) in ionocytes of living embryos.

Zebrafish embryos as an in vivo model to investigate cisplatin …

WebMar 6, 2013 · Cisplatin, one of the most commonly used anticancer drugs, is known to cause inner ear hair cell damage and hearing loss. Despite much investigation into mechanisms of cisplatin-induced hair cell death, little is known about the mechanism whereby cisplatin is selectively toxic to hair cells. WebFeb 15, 2024 · The cisplatin was used to produce a loss of CHCs to create a murine model of deafness. The AAV vectors were delivered into the scala media through the lateral wall. Compared with the control mice, the cisplatin-treated mice exhibited significantly enhanced apoptosis and autophagy in the cochleae, accompanied by a notably decreased Pou4f3 … simply southern clearance sale

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish ...

WebCisplatin is a very effective anticancer drug and generates reactive oxygen species (ROS) such as superoxide anions that can deplete antioxidant protective molecules in the cochlea. ... An in vivo study of transgenic zebrafish (Brn3C: EGFP) was used to investigate the protective effects of apocynin on cisplatin-induced hair cell death. In an in ... WebNational Center for Biotechnology Information WebCisplatin, an inorganic platinum derivative, is one of the most effective anticancer drugs and is widely used for the treatment of many malignancies, including brain, head and neck, lung, breast, and ovarian cancers [1][2]. Cisplatin acts by crosslinking purine bases in DNA. simply southern clearance shirts

Behavioral changes of zebrafish according to cisplatin-induced toxicity ...

WebInner ear sensory hair cells die following exposure to aminoglycoside antibiotics or chemotherapeutics like cisplatin, leading to permanent auditory and/or balance deficits in humans. Zebrafish (Danio rerio) are used to study drug-induced sensory hair cell death since their hair cells are similar in structure and function to those found in humans. WebConclusion: The zebrafish lateral line was used to identify 2 small molecules that protected against cisplatin-induced hair cell death. Key Words: CisplatinVHair cellVOtotoxicityV Protection. Otol Neurotol 36:519Y525, 2015. Cisplatin is a commonly used anticancer drug in the treatmentofmanycancers,includinglungcancer,ovarian ray white asset management shailer parkWebDec 1, 2024 · Cisplatin ototoxicity Zebrafish Mitochondrial dysfunction Mitochondrial bioenergetics 1. Introduction Cisplatin is a chemotherapeutic agent that is commonly used in the treatment of solid tumors. ray white aspley qld

"WebZebrafish may offer key insights to cisplatin ototoxicity because their lateral-line organ contains hair cells that are remarkably similar to those within the cochlea but are optically accessible, permitting observation of cisplatin injury in live intact hair cells. " - Cisplatin zebrafish

Cisplatin zebrafish

WebJan 1, 2024 · Exposure of zebrafish larvae (5dpf) to 1000 μM of cisplatin for 4 h induced extensive loss of hair cells in the neuromasts in the anterior, middle, and posterior regions ( Fig. 1 ). A similar loss of hair cells with a dose of cisplatin ranging from 250 to 1500 μM cisplatin was reported by Ou et al. (2007). WebMar 27, 2024 · Cisplatin is a platinum-based chemotherapy compound effective against a variety of cancers. However, it can cause increased reactive oxygen species (ROS) production in auditory and vestibular tissue leading to permanent hearing and balance loss. The amino acid, L-serine, has been shown to reduce ROS in some tissue types.

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WebFeb 6, 2014 · Zebrafish were exposed to 1000 μM cisplatin for 6 h. We, then, periodically monitored swimming depth, total swimming distance, peak swimming velocity, and mean swimming velocity of the fish for approximately 21 days. WebMay 15, 2024 · The zebrafish has appeared as an interesting model to study renal function, kidney regeneration, and injury, as renal structures conserve functional similarities with mammals. Adult zebrafish injected with cisplatin shows decreased survival, kidney cell death, and increased inflammation markers after 24 h post-injection (hpi).

WebMar 3, 2013 · Zebrafish were pretreated with cimetidine for 1 h, followed by cotreatment with 50 μ m cisplatin for an additional 24 h. We found no significant protection against cisplatin-induced hair cell loss for all concentrations of cimetidine evaluated ( p > 0.05, one-way ANOVA; Fig. 1a ). Figure 1. WebNov 1, 2007 · Five dpf zebrafish larvae, prelabeled with FM1-43FX were exposed to cisplatin at concentrations of 0, 0.25, 0.5, 0.75, 1, and 1.5 mM for 4 h. Doses were chosen empirically in pilot studies by identifying high doses of cisplatin that caused hair cell death after short durations without mortality of the zebrafish.

WebJan 1, 2024 · This study provides evidence that cisplatin-induced hair cell loss in zebrafish is accompanied by protein nitration and Lmo4 degradation, which are emerging as … WebOur findings demonstrate that cisplatin can alter the locomotion behavior of zebrafish. Behavioral changes of zebrafish according to cisplatin-induced toxicity of the balance …

WebNov 18, 2024 · Zebrafish have been used in many studies as an in vivo model to evaluate the cellular mechanisms of cisplatin-induced hair cell death and to identify potential … ray white aspley groupWebJul 7, 2024 · Using cisplatin to model CICI (), we performed RNA sequencing in the mouse hippocampus to identify novel molecular contributors and found profound up-regulation of the adenosine A 2A receptor (A 2A R) by cisplatin (Fig. 1A), potentially representing a mechanism for cisplatin-induced neurotoxicity.Adenosine maintains central nervous … simply southern clothing dogWebNov 18, 2024 · Cisplatin is an effective anticancer agent, but also causes permanent hearing loss by damaging hair cells—the sensory receptors essential for hearing. There is an urgent clinical need to protect cochlear hair cells in … simply southern clothes for girlsWebFeb 15, 2024 · Cisplatin or doxorubicin induces pyroptosis of renal tubular epithelial cells It has been reported that the typical characteristics of pyroptosis were increased LDH release, increased PI-positive... ray white asset rentalsWebFeb 18, 2024 · For this study, adult female zebrafish were given cisplatin (G2), the probiotic, Bacillus megaterium (G3), and cisplatin+ B. megaterium (G4) for 30 days, in addition to the control (G1). In order to investigate changes in antioxidative enzymes, ROS production, and histological changes after treatment, the intestines and ovaries were … simply southern clothes for menWebDec 1, 2024 · Zebrafish may offer key insights to cisplatin ototoxicity because their lateral-line organ contains hair cells that are remarkably similar to those within the cochlea but … ray white atherton real estateWebFeb 8, 2024 · Adult zebrafish injected with cisplatin shows decreased survival, kidney cell death, and increased inflammation markers after 24 h post-injection (hpi). In this model, … ray white atherton rentals