Webmitotic slippage reduced the sensitivity of the cells to microtu-bule-targeting agents, and cells that survived the microtubule- ... S3, HCT116, and NIH3T3, capable of inducing doxycycline (Dox)2 concentration-dependent expression of v-Src. None-theless, when genetic diversity is achieved, cells resistant to WebJul 1, 2005 · Temporal coupling of KSP-IA-induced apoptosis and mitotic slippage in HCT116 cellsCells were synchronized at G1/S boundary by double thymidine block. Upon release from the block (0 hr), cells were treated with either vehicle or KSP-IA (300 nM) for various times. Cells, collected immediately after drug treatment, were analyzed for DNA …
Polyploidy formation in cancer cells: How a Trojan horse is born
WebMar 16, 2024 · The mitotic indices of HCT116 cells with and without APC-ΔC expression treated with 100 nM BI6727 peaked at 20–24 h. ... Previous studies indicated that mitotic slippage occurs via slow Cyclin ... WebDownload scientific diagram APC substrate degradation upon mitotic slippage with different concentrations of nocodazole. (A) Asynchronous HCT116 cells were treated with 60, 200 and 500... howard house newport isle of wight
Substrate degradation by the anaphase promoting …
WebHCT116 cells were examined by immunofluorescence confocal microscopy. Cells were treated with 500 nM AK301 for 16 hours, and then processed for γH2AX and γ-tubulin staining (A) or Aurora B and... WebMay 19, 2016 · Microtubule poisons inhibit spindle function, leading to activation of spindle assembly checkpoint (SAC) and mitotic arrest. Cell death occurring in prolonged mitosis is the first target of microtubule poisons in cancer therapies. However, even in the presence of microtubule poisons, SAC and mitotic arrest are not permanent, and the surviving cells … WebCancer cells exit from drug-induced mitotic arrest (mitotic slippage) to avoid subsequent cell death which is thought to be a major mechanism contributing to this resistance. The … howard houser strongsville ohio